Reagent Compatibility
His tag OneStep ELISA Kit
His tag OneStep ELISA Kit
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$500 USD
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$500 USD
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Product Details
Product Details
Product Specification
| Antigen | His tag |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Species Independent |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Sample type | N-terminal/C-terminal/internal His-tagged proteins. Mammalian and bacteria cell lysates or cell supernatant. |
| Assay type | Competitive (quantitative) |
| Sensitivity | 4 ng/ml |
| Range | 4 ng/ml -1000 ng/ml |
| Assay time | 60 minutes |
Background
A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues. Histidine (His)-tag is widely used for affinity purification of recombinant proteins, but the yield and purity of expressed proteins are quite different. This kit is developed for rapid and high throughput detection of His-tagged proteins.
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ELISA
Protocol Diagram
