1μg (R: reducing condition, N: non-reducing condition).
Glypican 1/GPC1(S486A, S488A, S490A) His Tag Protein, Human
Glypican 1/GPC1(S486A, S488A, S490A) His Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | FLJ38078; Glypican 1; glypican proteoglycan 1; glypican; glypican-1; GPC1 |
| Accession | P35052-1 |
| Amino Acid Sequence | Asp24-Ser530 with His Tag at C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 55-72kDa (Reducing) |
| Purity | > 95% by SDS-PAGE & SEC-HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, PH7.4,5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1.Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241. |
Background
Glypicans, a small multigene family of glycosylphosphatidylinositol (GPI)-linked proteoglycans, play a pivotal role in growth factor signaling. GPC-1, a member of the glypican family, is expressed in various cell types including neurons, smooth and skeletal muscle cells, keratinocytes, osteoblasts, Schwann cells, immature dendritic cells, tumor cells, and tumor-associated vascular endothelial cells. The glypican-related integral membrane proteoglycan family (GRIPS) consists of core proteins anchored to the plasma membrane via a glycosyl phosphatidylinositol linkage. These proteins are implicated in the regulation of cell division and growth control.
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SDS-PAGE
SEC-HPLC
The purity of Glypican 1/GPC1(S486A, S488A, S490A) His Tag Protein, Human is more than 95 % as determined by SEC-HPLC.
