1μg (R: reducing conditions, N: non-reducing conditions).
Biotinylated IL-4R alpha/CD124 Avi&His Tag Protein, Human
Biotinylated IL-4R alpha/CD124 Avi&His Tag Protein, Human
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$560 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | IL4R, CD124, IL4RA |
| Accession | P24394 |
| Amino Acid Sequence | Met26-His232, with C-terminal Avi Tag&His Tag MKVLQEPTCVSDYMSISTCEWKMNGPTNCSTELRLLYQLVFLLSEAHTCIPENNGGAGCVCHLLMDDVVSADNYTLDLWAGQQLLWKGSFKPSEHVKPRAPGNLTVHTNVSDTLLLTWSNPYPPDNYLYNHLTYAVNIWSENDPADFRIYNVTYLEPSLRIAASTLKSGISYRARVRAWAQCYNTTWSEWSPSTKWHNSYREPFEQHGGGSGLNDIFEAQKIEWHEHHHHHHHH |
| Expression System | HEK293 |
| Molecular Weight | 40-50kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Biotin |
| Tag | Avi Tag, His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Keats Nelms, THE IL-4 RECEPTOR: Signaling Mechanisms and Biologic Functions. Annu. Rev. Immunol. 1999. 17:701–38. |
Background
IL-4Rα is a member of the hematopoietin receptor superfamily. Among the defining features of the members of this superfamily of receptors are shared structural motifs in the extracellular region, which consists of type III fibronectin domains. These motifs include conserved paired Cys residues and, in the membrane proximal region, a WSXWS motif. The latter has been proposed to be required for maintaining the receptor in a conformation favorable to cytokine binding. Structural alterations in the IL-4Rα extracellular region may result in altered receptor signaling capabilities. Indeed, a variant of the human IL-4Rα chain containing a Ile50Val substitution was isolated from atopic individuals and has been shown to enhance signal transduction resulting in the increased production of IgE.
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SDS-PAGE
ELISA
Immobilized IL-4, Human (Cat. No. UA040026) at 1.5μg/mL (100μL/well) can bind Biotinylated IL-4R alpha/CD124 Avi&His Tag, Human (Cat. No. UA010671) with EC50 of 12.51-16.32ng/mL.
Immobilized Biotinylated IL-4R alpha/CD124 Avi&His Tag, Human (Cat. No. UA010671) at 2.0μg/mL (100μL/well) can bind Dupilumab (Cat. No. UA010946) with EC50 of 5.78-7.91 ng/mL.
Immobilized IL-4 Protein, Human (Cat. No. UA040026) at 5.0μg/mL (100μL/well) can bind Biotinylated IL-4R alpha/CD124 Avi&His Tag Protein, Human (Cat. No. UA010671) with EC50 of 27.67-45.87ng/mL.
