Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D, The EC50 for this effect is less than 1ng/ml.
TRAIL Protein, Mouse
TRAIL Protein, Mouse
Price:
Regular price
$144 USD
Regular price
Sale price
$144 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | TNF-related apoptosis-inducing ligand; Protein TRAIL; CD_antigen: CD253; Tnfsf10 |
| Accession | P50592 |
| Amino Acid Sequence | Arg119-Asn291 |
| Expression System | E.coli |
| Molecular Weight | 19kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE and HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 300mM NaCl, 1mM DTT, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Andrew Thorburn (2007) J Thorac Oncol. 2(6):461-5. |
Background
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), also known as CD253 or TNFSF10, can be produced and secreted by most normal tissue cells. TRAIL proteins are available in two forms: membrane-bound (full-length TRAIL) and soluble (sTRAIL). In vivo, only the membrane-bound form exists, while the soluble polypeptide (sTRAIL), which is formed in the extracellular region of TRAIL, has the full biological activity of the membrane-bound TRAIL. Both of them are able to bind to specific receptors on the cell membrane and rapidly induce the expression of TRAIL through intracellular signal transduction. They can rapidly induce apoptosis in cells expressing TRAIL-specific receptors. TRAIL has a broad spectrum of action and can induce apoptosis in a variety of tumor cells in the colon, lung, breast, kidney, brain and skin, and is considered as a promising broad-spectrum anti-cancer molecule.
Picture
Picture
Bioactivity
SDS-PAGE
1μg (R: reducing condition, N: non-reducing condition).
SEC-HPLC
>95% as determined by SEC-HPLC.
