| Species | Human |
| Synonyms | TNFα;Tumor Necrosis Factor, Cachectin, TNF-Alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, TNF, TNFA, TNFSF2 |
| Accession | P01375 |
| Amino Acid Sequence | Val77-Leu233 |
| Expression System | CHO |
| Molecular Weight | 17 kDa(Reducing) |
| Purity | ＞95%, by SDS-PAGE under reducing conditions |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation . |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
Tumor necrosis factor alpha (TNFa) is a multifunctional proinflammatory cytokine synthesized mainly by nucleated blood
cells as a 233 aa type II transmembrane protein which is cleaved by ADAM17 between aa 76-77 to form a soluble
homotrimeric complex. TNFa plays a role in lipid metabolism, coagulation, and endothelial function and has been
associated with cancer, infection and inflammation (including inflammatory bowel disease), ischemia/reperfusion injury
and heart failure, and insulin resistance.
Primary antibody: Phospho-NF-κB p65 (Ser536) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa treated with 100 ng/ml Calyculin A for 30 minutes, whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes and 20 ng/ml TNF-α for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 65 kDa
Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, EC50 < 40pg/ml.
Anti-His antibody Immobilized on CM5 Chip captured TNFR-1/CD120a His Tag, Human (Cat. No. UA040028), can bind TNF-α, Human (Cat. No. UA040005) with an affinity constant of 0.106nM as determined in SPR assay.
Anti-His antibody Immobilized on CM5 Chip captured TNFR-2/CD120b His Tag, Human (Cat. No. UA010138), can bind TNF-α, Human (Cat. No. UA040005) with an affinity constant of 1.53 nM as determined in SPR assay.
