IL-2 Protein, Human (GMP)
IL-2 Protein, Human (GMP)
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; |
| Accession | P60568 |
| Amino Acid Sequence | Ala21-Thr153 |
| Expression System | E.coli |
| Molecular Weight | 15±2kD (Reducing) |
| Purity | >95% by SDS-PAGE and HPLC |
| Endotoxin | ≤10 EU/mg |
| Host cell protein | ＜100ppm |
| Host cell DNA | ＜100ppb |
| Mycoplasma | 阴性 |
| Sterility | 符合 |
| phValue | 7.1~7.7 |
| ProteinContent | 90%~120% |
| SdsPageMolecularWeight | 15±2kD（还原） |
| SdsPagePurity | ≥95%（非还原） |
| RpHplcPurity | ≥95% |
| SecHplcPurity | ≥95% |
| CellActivity | ＞5.00 x 10^6 IU/mg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, 5% trehalose pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1. J Exp Med. 2020 Jan 6;217(1): e20191247. |
Background
Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotropic hormone that is instrumental in the body natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-Regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self-antigens", which could result in "autoimmunity". T-Regs do this by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.
