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IGF-I Protein, Mouse

IGF-I Protein, Mouse

Catalog Number: UA040099 Reactivity: Mouse Conjugation: Unconjugated Brand: UA BIOSCIENCE
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Regular price $99 USD
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Product Details

Product Specification


Species Mouse
Antigen IGF-1
Synonyms IGF-I Protein, IGF1A Protein, IGFI Protein
Accession P05017
Amino Acid Sequence Gly49-Ala118
Expression System E.coli
Molecular Weight

8-9kDa (Non-Reducing)

Purity

>95% by SDS-PAGE

Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tag No Tag
Physical Appearance Lyophilized Powder
Storage Buffer 50mM NaAc, pH6.0.
Reconstitution Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1.Bartlett WP,Li XS, Williams M. 1992. Brain Res Mol Brain Res, 12: 285-91.


Background

Insulin-like growth factor I (IGF-I) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor protein containing N-terminal and C-terminal propeptide regions. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. IGF1 binds IGF-1R, IGF-2R, and the insulin receptor and plays a key role in cell cycle progression, cell proliferation and tumor progression.

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Bioactivity

Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The EC50 for this effect is less than 5ng/ml.

SDS-PAGE

1μg (R: reducing condition, N: non-reducing condition).

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