2μg (R: reducing condition, N: non-reducing condition).
Flt-3/Flk-2 Protein, Human
Flt-3/Flk-2 Protein, Human
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$120 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Receptor-type tyrosine-protein kinase FLT3; FL cytokine receptor; Fetal liver kinase-2 (FLK-2); Fms-like tyrosine kinase 3 (FLT-3); Stem cell tyrosine kinase 1 (STK-1); |
| Accession | P36888 |
| Amino Acid Sequence | Asn27-Asn541, with C-terminal hIgG1 Fc. |
| Expression System | HEK293 |
| Molecular Weight | 100-120 kDa (Reducing) |
| Purity | >95% by SDS-PAGE and HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | Human IgG1 Fc |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | Immunity. 2002 Oct;17(4):463-72. |
Background
Fms-like tyrosine kinase 3 (Flt-3), also known as fetal liver kinase 2 (Flk-2), is a receptor tyrosine kinase that plays a critical role in hematopoiesis and immune cell development. Flt-3 is primarily expressed on hematopoietic stem cells and early progenitor cells, where it regulates cell proliferation, differentiation, and survival. Activation of Flt-3 by its ligand, Flt-3 ligand (Flt-3L), leads to the activation of downstream signaling pathways involved in hematopoietic cell growth and differentiation. Flt-3 is essential for the development of various immune cell lineages, including dendritic cells, natural killer cells, and B cells.
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SDS-PAGE
SEC-HPLC
>95% as determined by SEC-HPLC.
ELISA
Measured by its binding ability in a functional ELISA. When Recombinant Human FLT3L is immobilized 2.5µg/mL (100 µl/well), Recombinant Human FLT3/FLK2 Fc binds with an EC50 of 0.007-0.012 ug/ml.
