Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells. The EC50 for this effect is less than 10ng/ml.
FGF-12 Protein, Mouse/Rat
FGF-12 Protein, Mouse/Rat
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | Fibroblast growth factor 12, FGF-12, Fibroblast growth factor homologous factor 1 (FHF-1), Myocyte-activating factor |
| Accession | P61329 |
| Amino Acid Sequence | Met1-Thr243 |
| Expression System | E.coli |
| Molecular Weight | 27kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 500mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | Wiley Interdiscip Rev Dev Biol. 2015 May-Jun;4(3):215-66. Epub 2015 Mar 13. |
Background
Members of the FGF family function in the earliest stages of embryonic development and during organogenesis to maintain progenitor cells and mediate their growth, differentiation, survival, and patterning..Among the FGF proteins, the least characterized superfamily is the group of fibroblast growth factor homologous factors (FHFs). The fibroblast growth factor (FGF) family includes 22 genes found in humans that share sequence and structural similarity. FGFs fall into three major groups: canonical FGFs (comprising subfamilies 1, 4, 7, 8 and 9), endocrine FGFs, referred to as the FGF19 subfamily, and fibroblast growth factor homologous factors (FHFs), until recently considered intracellular FGFs, termed the FGF11 subfamily.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
