WB result of SYT2 Rabbit pAb
Primary antibody: SYT2 Rabbit pAb at 1/1000 dilution
Lane 1: TT whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 46-75 kDa
SYT2 Rabbit Polyclonal Antibody
SYT2 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SYT2 |
| Synonyms | Synaptotagmin-2; Synaptotagmin IIImported (SytII) |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Synapse |
| Accession | Q8N9I0 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | TT, mouse cerebellum, rat cerebellum |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20°C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Ms, Rt |
Background
Synaptotagmin-2 (SYT2) is a synaptic vesicle membrane protein that functions as a calcium sensor and plays a crucial role in fast Ca²⁺ -dependent neurotransmitter release at the presynaptic nerve terminal. It is widely expressed in the brain and is particularly important at the neuromuscular junction. SYT2 contains two calcium-binding domains, C2A and C2B, with the C2B domain being essential for mediating vesicle fusion and neurotransmitter release. Mutations in the SYT2 gene can lead to various neuromuscular disorders, such as distal motor neuropathy and myasthenic syndrome, which are often associated with presynaptic dysfunction at the neuromuscular junction. Recent studies have also shown that SYT2 can serve as a reliable marker for analyzing the development and plasticity of inhibitory synapses formed by parvalbumin-positive interneurons in the visual cortex.
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Picture
Western Blot
WB result of SYT2 Rabbit pAb
Primary antibody: SYT2 Rabbit pAb at 1/1000 dilution
Lane 1: mouse cerebellum lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 46-75 kDa
WB result of SYT2 Rabbit pAb
Primary antibody: SYT2 Rabbit pAb at 1/1000 dilution
Lane 1: rat cerebellum lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 46-75 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti-SYT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse cardiac muscle. Anti-SYT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebellum. Anti-SYT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
