WB result of p120 Catenin Rabbit mAb Primary antibody: p120 Catenin Rabbit mAb at 1/1000 dilution Lane 1: HL-60 whole cell lysate 20 µg Lane 2: HT-29 whole cell lysate 20 µg Lane 3: HeLa whole cell lysate 20 µg Lane 4: A431 whole cell lysate 20 µg Negative control: HL-60 whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 108 kDa Observed MW: 70~105 kDa
p120 Catenin Recombinant Rabbit mAb (SDT-350-75)
p120 Catenin Recombinant Rabbit mAb (SDT-350-75)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | p120 Catenin |
| Synonyms | Catenin delta-1; Cadherin-associated Src substrate; CAS; p120 catenin; p120(ctn); p120(cas) |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane, Cytoplasm |
| Accession | O60716 |
| Clone Number | SDT-350-75 |
| Antibody Type | Recombinant mAb |
| Application | ICFCM, IHC-P, ICC, WB, IF |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC | 1:500-1:1000 | |
| ICC | 1:1000 | |
| IF | 1:500 | |
| ICFCM | 1:500 |
Background
Catenin δ-1 (P120 Catenin) belongs to the β-serial protein family .p-120 catenin (along with α, β and γ catenins) connects the transmembrane protein E-cadherin to the actin cytoskeleton in the cell cytoplasm. It expresses in various normal epithelial tissues (including thyroid, breast, stomach, duodenal, liver, pancreas, colon, kidney, prostate, testicles), various non -epithelial tissues (including myocardial cells, neurons, gel cells To. p-120 regulates the transformation of calcium adhesion protein on the surface of the cells, thereby determining the E-calcium adhesive protein levels used in the cells, and then plays an important role in the adhesion of the cells. Many studies have shown that p-120 irregularly expression or lack of disobedience in a variety of cancer cells indicates that p-120 play a role in cancer-suppressing genes.
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Western Blot
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HL-60 (Human acute promyelocytic leukemia promyeloblast, left) / HT29 (Human colorectal adenocarcinoma epithelial cell, Right) cells labelling E-Cadherin antibody at 1/500 dilution (0.1 μg) / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: HL-60
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat esophagus. Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human invasive lobular breast cancer. Anti-p120 Catenin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HT-29 cells. Anti-p120 Catenin antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Negative control: ICC shows negative staining in HL-60 cells. Anti-p120 Catenin antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
