WB result of Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb
Primary antibody: Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HepG2 whole cell lysate 20 µg
Lane 2: HepG2 treated with 100 ng/ml EGF for 20 minutes whole cell lysate 20 µg
Lane 3: untreated HeLa whole cell lysate 20 µg
Lane 4: HeLa treated with 60 µg/ml TPA for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 83 kDa
Observed MW: 90 kDa
Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb (S-985-212)
Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb (S-985-212)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-p90 RSK (T359/S363) |
| Synonyms | Ribosomal protein S6 kinase alpha-1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1 (p90-RSK 1; p90RSK1; p90S6K); MAP kinase-activated protein kinase 1a (MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a); Ribosomal S6 kinase 1 (RSK-1); RPS6KA1; MAPKAPK1A; RSK1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q15418 |
| Clone Number | S-985-212 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HepG2, HeLa |
| Predicted Reactivity | Ck, Xe |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu,Ms,Rt |
Background
p90 RSK, also known as RSK1, is a member of the 90 kDa ribosomal S6 kinase family, which includes RSK1, RSK2, and RSK3. These are serine/threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2. RSK1 is activated by MAPK in vitro and in vivo via phosphorylation. Active RSKs play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB, which are key regulators of gene expression. The phosphorylation of RSK1 at T359/S363 is part of the signaling cascade initiated by growth factors and hormones, which leads to cellular proliferation and survival.
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Picture
Western Blot
WB result of Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb
Primary antibody: Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 treated with 100 ng/ml EGF for 20 minutes whole cell lysate 20 µg
Lane 2: HeLa treated with 60 µg/ml TPA for 30 minutes whole cell lysate 20 µg
Lane 3: HepG2 treated with 100 ng/ml EGF for 20 minutes, then treated with phosphatase whole cell lysate 20 µg
Lane 4: HeLa treated with 60 µg/ml TPA for 30 minutes, then treated with phosphatase whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 83 kDa
Observed MW: 90 kDa
Dot Blot
Dot blot result of Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb
Lane 1: p90 RSK (T359/S363) phospho peptide
Lane 2: p90 RSK (S363) phospho peptide
Lane 3: p90 RSK (T359) phospho peptide
Lane 4: p90 RSK unmodified peptide
Primary antibody: Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
