Flow cytometric analysis of Mouse CD19 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes cells were stained with Alexa Fluor® 647 Rat Anti-Mouse CD3 antibody and either PerCP-Cy5.5 Rat IgG2a, κ Isotype Control (Left panel) or SDT PerCP-Cy5.5 Rat Anti-Mouse CD19 antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PerCP-Cy5.5 Rat Anti-Mouse CD19 Antibody (S-R532)
PerCP-Cy5.5 Rat Anti-Mouse CD19 Antibody (S-R532)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Mouse CD19 |
| Synonyms | B-lymphocyte antigen CD19; Differentiation antigen CD19 |
| Location | Cell membrane |
| Accession | P25918 |
| Clone Number | S-R532 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.05mg/ml |
| Conjugation | PerCP-Cy5.5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Ms |
Background
CD19 is a 95 kd transmembrane glycoprotein that belongs to the immunoglobulin superfamily. It is a type I transmembrane protein with a single transmembrane domain, a cytoplasmic C-terminus, and an extracellular N-terminus. CD19 is crucial for B cell development and signaling as it modulates both B cell receptor (BCR)-dependent and independent signaling pathways. It is involved in the humoral immune response and tolerance induction, functioning as a critical co-receptor that amplifies the activation of Src-family protein tyrosine kinases.
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