Flow cytometric analysis of Human CD18 expression on Human PBMC. Human PBMC (peripheral blood mononuclear cells) were stained with Brilliant Violet 421™ Mouse Anti-Human CD14 and either PE-Cy7 Mouse IgG1, κ Isotype Control (Left panel) or SDT PE-Cy7 Mouse Anti-Human CD18 Antibody (Right panel) at 1.25 μl/test treated with True-Stain Monocyte Blocker™. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PE-Cy7 Mouse Anti-Human CD18 Antibody (S-R487)
PE-Cy7 Mouse Anti-Human CD18 Antibody (S-R487)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD18 |
| Synonyms | Integrin beta-2; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; MFI7; ITGB2 |
| Location | Cell membrane |
| Accession | P05107 |
| Clone Number | S-R487 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PE-Cy7 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
CD18, also known as integrin β2, is a 95 kDa glycoprotein that is a crucial component of several β2 integrins. These integrins are heterodimeric type I transmembrane proteins composed of a variable alpha chain (CD11a-d) and the common beta chain CD18. The CD18 protein is encoded by the ITGB2 gene in humans. It plays a significant role in cellular adhesion and immune responses. When CD18 binds with different alpha chains, it forms various heterodimers such as lymphocyte function-associated antigen-1 (LFA-1) with CD11a, and Macrophage-1 antigen receptor (Mac-1) with CD11b. These heterodimers are involved in processes like leukocyte adhesion to endothelial cells, phagocytosis, and immune cell activation. Deficiencies in CD18 expression can lead to adhesion defects in white blood cells, impairing the immune system's ability to fight infections.
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