IHC shows positive staining in paraffin-embedded human kidney. Anti-NPHS2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
NPHS2 Recombinant Rabbit mAb (S-1824-36)
NPHS2 Recombinant Rabbit mAb (S-1824-36)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NPHS2 |
| Synonyms | Podocin |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q9NP85 |
| Clone Number | S-1824-36 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P |
| Reactivity | Hu |
| Positive Sample | Human kidney |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:500 | Hu |
Background
NPHS2 protein, also known as podocin, is a key component of the slit diaphragm in the glomerular filtration barrier and plays a crucial role in regulating glomerular permeability; mutations in this protein can lead to steroid-resistant nephrotic syndrome.
Picture
Picture
Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-NPHS2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
