Flow cytometric analysis of 4% PFA fixed 0.1% Tween-20 permeabilized PBMC (Human peripheral blood mononuclear cells) labelling Granzyme B antibody at 1/200 dilution (1 μg)/ (Right panel) compared with a Mouse IgG1, κ Isotype Control (Left panel). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Mouse Anti-Human/Mouse Granzyme B Antibody (S-2865)
Mouse Anti-Human/Mouse Granzyme B Antibody (S-2865)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Granzyme B |
| Synonyms | C11; CTLA-1; Cathepsin G-like 1 (CTSGL1); Cytotoxic T-lymphocyte proteinase 2 (Lymphocyte protease); Fragmentin-2; CGL1; CSPB; CTLA1; GRB; GZMB |
| Location | Secreted |
| Accession | P10144、P04187 |
| Clone Number | S-2865 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | ICFCM |
| Reactivity | Hu, Ms |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1:200 | Hu, Ms |
Background
Granzyme B is a 32-kDa serine protease stored in the cytolytic granules of cytotoxic T lymphocytes and natural killer cells, whose immune function centers on perforin-assisted translocation into target cells where it directly cleaves and activates pro-apoptotic substrates such as procaspase-3, Bid and PARP, thereby igniting both caspase-dependent and caspase-independent mitochondrial apoptosis pathways to eliminate virus-infected or malignant cells.
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