Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cells) labeling Human CD3d at 1/2000 (0.1 μg) dilution / (Right panel) compared with a Mouse IgG, Isotype Control / (Left panel). Goat Anti – Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD19 - Alexa Fluor® 647 separately. Gated on total viable cells.
Mouse Anti-Human CD3d Antibody (S-R565)
Mouse Anti-Human CD3d Antibody (S-R565)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD3d |
| Synonyms | T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain; T3D |
| Location | Cell membrane |
| Accession | P04234 |
| Clone Number | S-R565 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1:2000 | Hu |
Background
CD3d, or the CD3 delta subunit of the T-cell receptor complex, is a crucial component of the T-cell receptor (TCR) complex. The TCR/CD3 complex, including CD3d, is essential for signal transduction in T-cells. When the TCR recognizes and binds to an antigen presented by MHC molecules, the CD3 complex, including CD3d, transduces the activation signal into the T-cell. Defects in the CD3d can lead to severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK), highlighting the importance of CD3d in the immune system.
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