WB result of JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 25 μg/ml Anisomycin for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48, 53 kDa
Observed MW: 40, 53 kDa
This blot was developed with high sensitivity substrate
JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) Recombinant Rabbit mAb (S-3593)
JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) Recombinant Rabbit mAb (S-3593)
Price:
Regular price
$131.00 SGD
Regular price
Sale price
$131.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) |
| Synonyms | Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; JNK-46; Stress-activated protein kinase 1c (SAPK1c); Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; PRKM8; SAPK1; SAPK1C; MAPK8; Mitogen-activated protein kinase 9; MAP kinase 9; MAPK 9; JNK-55; Stress-activated protein kinase 1a (SAPK1a); Stress-activated protein kinase JNK2; c-Jun N-terminal kinase 2; MAPK9; PRKM9; SAPK1A; Mitogen-activated protein kinase 10; MAP kinase 10; MAPK 10; MAP kinase p49 3F12; Stress-activated protein kinase 1b (SAPK1b); Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3; MAPK10; JNK3A; PRKM10; SAPK1B |
| Location | Cytoplasm, Nucleus |
| Accession | P45983、P45984、P53779 |
| Clone Number | S-3593 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| ICC | 1:100 | Ms |
Background
JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) refers to the activated state of the three main isoforms of the c-Jun N-terminal Kinase family—JNK1, JNK2, and JNK3—following the phosphorylation of key residues within their activation loops. Specifically, the phosphorylation of threonine 183 (T183) in JNK1 and JNK2, and threonine 221 (T221) in JNK3, in conjunction with the phosphorylation of their respective tyrosine residues, is essential for the full activation of JNK. This dual phosphorylation induces a conformational change in the JNK protein, exposing its kinase active site. The JNK signaling pathway is a central hub for cellular responses to environmental stresses, such as ultraviolet radiation, oxidative stress, osmotic shock, and inflammatory cytokines. Upon activation, the phosphorylated JNK translocates into the nucleus, where it regulates critical cellular processes including proliferation, differentiation, apoptosis, and inflammatory responses by phosphorylating downstream transcription factors like c-Jun and ATF2. It is noteworthy that JNK1 and JNK2 are ubiquitously expressed across various tissues, whereas JNK3 is primarily found in the brain, heart, and testes and is closely associated with neuronal apoptosis. Consequently, detecting the phosphorylation levels of JNK at the T183/T221 sites is a key molecular indicator for measuring the activity of this pathway, and its aberrant activation is closely linked to the pathogenesis and progression of neurodegenerative diseases, cancer, insulin resistance, and various autoimmune disorders.
Picture
Picture
Western Blot
WB result of JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 25 μM Anisomycin for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48, 53 kDa
Observed MW: 40, 53 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in NIH/3T3 cells treated with Anisomycin (250ng/ml 30min). Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows weak staining in NIH/3T3 cells. Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows negative staining in lambda phosphatase treated NIH/3T3 cells. Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody was used at 1/100 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
