WB result of Histone H3 (tri methyl K9) Recombinant Rabbit mAb
Primary antibody: Histone H3 (tri methyl K9) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Histone H3 (tri methyl K9) Recombinant Rabbit mAb (S-3826)
Histone H3 (tri methyl K9) Recombinant Rabbit mAb (S-3826)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Histone H3 (tri methyl K9) |
| Synonyms | H3K9me3 |
| Location | Nucleus |
| Accession | P68431 |
| Clone Number | S-3826 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, NIH/3T, PC-12, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
Background
Histone H3 tri-methylated lysine 9 (H3K9me3) is a crucial epigenetic mark formed by the trimethylation of the ninth lysine residue on the core histone H3. It is widely recognized as a classic hallmark of constitutive heterochromatin. Its primary function is to recruit effector proteins like Heterochromatin Protein 1 (HP1), which subsequently promotes the compaction of chromatin into a dense, transcriptionally silent higher-order structure. This modification plays a key role in maintaining genomic stability by effectively repressing the aberrant expression of repetitive genomic elements, such as transposons and telomeric regions, as well as foreign genes. Furthermore, H3K9me3 is essential for the transcriptional regulation of specific genes, X-chromosome inactivation, and cell fate determination. It is important to note that aberrant distribution of H3K9me3 is closely associated with various diseases, including cancer development, neurodegenerative disorders, and the aging process.
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Western Blot
WB result of Histone H3 (tri methyl K9) Recombinant Rabbit mAb
Primary antibody: Histone H3 (tri methyl K9) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
WB result of Histone H3 (tri methyl K9) Recombinant Rabbit mAb
Primary antibody: Histone H3 (tri methyl K9) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
WB result of Histone H3 (tri methyl K9) Recombinant Rabbit mAb
Primary antibody: Histone H3 (tri methyl K9) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-Histone H3 (tri methyl K9) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Histone H3 (tri methyl K9) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-Histone H3 (tri methyl K9) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-Histone H3 (tri methyl K9) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-Histone H3 (tri methyl K9) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
