WB result of Histone H3 (di methyl K79) Recombinant Rabbit mAb
Primary antibody: Histone H3 (di methyl K79) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Histone H3 (di methyl K79) Recombinant Rabbit mAb (S-861-22)
Histone H3 (di methyl K79) Recombinant Rabbit mAb (S-861-22)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Histone H3 (di methyl K79) |
| Synonyms | H3K79me2 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P68431 |
| Clone Number | S-861-22 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HepG2, Jurkat, Neuro-2a, C2C12, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
Background
Histone H3 (di-methyl K79), or H3K79me2, is an epigenetic modification where lysine 79 on the histone H3 protein is dimethylated. This mark is primarily deposited by the methyltransferase DOT1L and plays a crucial role in transcriptional regulation, DNA damage repair, and cell cycle progression. H3K79me2 is associated with active gene transcription, particularly at promoter and enhancer regions, and is implicated in maintaining chromatin structure during development and differentiation. Dysregulation of H3K79 methylation is linked to cancers such as leukemia, where aberrant DOT1L activity drives oncogenic gene expression. Additionally, H3K79me2 serves as a recognition site for chromatin-binding proteins, influencing processes like DNA replication and stem cell maintenance. Its dynamic regulation makes it a potential therapeutic target in epigenetic-based diseases.
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Western Blot
WB result of Histone H3 (di methyl K79) Recombinant Rabbit mAb
Primary antibody: Histone H3 (di methyl K79) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: Neruo-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
WB result of Histone H3 (di methyl K79) Recombinant Rabbit mAb
Primary antibody: Histone H3 (di methyl K79) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Dot Blot
Dot blot result of Histone H3 (di methyl K79) Recombinant Rabbit mAb
Lane 1: H3K79me2 peptide
Lane 2: H3K79me1 peptide
Lane 3: H3K79me3 peptide
Lane 4: H3K79un peptide
Primary antibody: Histone H3 (di methyl K79) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- Histone H3 (di methyl K79) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- Histone H3 (di methyl K79) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
