WB result of DLX5 Recombinant Rabbit mAb
Primary antibody: DLX5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: U-87 MG whole cell lysate 20 µg
Lane 3: U-2 OS whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Lane 5: A375 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 37 kDa
DLX5 Recombinant Rabbit mAb (S-2785-79)
DLX5 Recombinant Rabbit mAb (S-2785-79)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | DLX5 |
| Synonyms | Homeobox protein DLX-5 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P56178 |
| Clone Number | S-2785-79 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, U-87 MG, U-2 OS, A549, A375, mouse placenta, rat placenta |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
| IF | 1:500 | Ms, Rt |
Background
DLX5 is a distal-less homeobox transcription factor essential for embryonic development, particularly in osteogenesis, chondrogenesis, and craniofacial patterning, where it activates Runx2 and Sp7 to drive osteoblast differentiation while also regulating chondrocyte hypertrophy and coupling bone formation to resorption by modulating the RANKL/OPG ratio in mesenchymal cells; it binds the 5'-TAATTA-3' consensus, synergizes with BMP signaling, and its human mutations are linked to split-hand/split-foot malformation, sensorineural hearing loss, and enhanced osteosarcoma progression via NOTCH pathway activation.
Picture
Picture
Western Blot
WB result of DLX5 Recombinant Rabbit mAb
Primary antibody: DLX5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse placenta lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 37 kDa
WB result of DLX5 Recombinant Rabbit mAb
Primary antibody: DLX5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat placenta lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 37 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-DLX5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-DLX5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-DLX5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-DLX5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-DLX5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-DLX5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in U-87MG cells. Anti-DLX5 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-DLX5 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat cerebral cortex. Anti-DLX5 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
