WB result of Cardiac troponin T (TNNT2) Recombinant Rabbit mAb
Primary antibody: Cardiac troponin T (TNNT2) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Negative control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 39 kDa
Cardiac troponin T (TNNT2) Recombinant Rabbit mAb ,PBS Only (SDT-2894-34)
Cardiac troponin T (TNNT2) Recombinant Rabbit mAb ,PBS Only (SDT-2894-34)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Cardiac troponin T (TNNT2) |
| Synonyms | Troponin T, cardiac muscle; TnTc; Cardiac muscle troponin T (cTnT) |
| Immunogen | Recombinant Protein |
| Accession | P45379 |
| Clone Number | SDT-2894-34 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Human heart, mouse heart, rat heart |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 4 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:250 | Hu |
Background
Cardiac troponin T (TNNT2) is a 35.9 kDa, 295-amino-acid striated-muscle-specific protein that forms the troponin T component of the troponin complex on the thin filament, where it tethers the troponin C–I sub-complex to tropomyosin; by transducing the Ca²⁺-induced conformational change of troponin C into tropomyosin movement, TNNT2 orchestrates the steric switch that exposes myosin-binding sites on actin and thus initiates systolic contraction, while its unique N-terminal peptide sequence allows ultra-sensitive immunoassay detection—making any elevation in blood TNNT2 the gold-standard biomarker for myocardial injury—yet mutations that enhance or suppress myofilament Ca²⁺ sensitivity, destabilize the N-tail, or promote aberrant splice variants are linked to hypertrophic, dilated, or restrictive cardiomyopathies, arrhythmogenic right ventricular cardiomyopathy, and sudden cardiac death, underscoring its dual role as both a molecular motor controller and a sentinel of cardiac integrity.
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Picture
Western Blot
WB result of Cardiac troponin T (TNNT2) Recombinant Rabbit mAb
Primary antibody: Cardiac troponin T (TNNT2) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat skeletal muscle lysate 20 µg
Lane 2: rat heart lysate 20 µg
Negative control: rat skeletal muscle lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 39 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Cardiac troponin T (TNNT2) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-Cardiac troponin T (TNNT2) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-Cardiac troponin T (TNNT2) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-Cardiac troponin T (TNNT2) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human cardiac muscle. Anti- Cardiac troponin T (TNNT2) antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded human skeletal muscle. Anti- Cardiac troponin T (TNNT2) antibody was used at 1/250 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
