IHC shows positive staining in paraffin-embedded human heart muscle. Anti-ZO-1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ZO-1 Recombinant Rabbit mAb
ZO-1 Recombinant Rabbit mAb
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ZO-1 |
| Synonyms | TJP1; tight junction protein 1 |
| Location | Cell membrane, Cell junction |
| Accession | Q07157 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:100-1:200 |
Background
ZO-1 (zonula occludens-1) protein is a member of the membrane-associated guanylate kinase (MAGUK) family and a key component of tight junctions, which are crucial for maintaining the integrity and permeability of epithelial and endothelial barriers. It contains three PDZ domains, a SH3 domain, and a guanylate kinase (GUK) domain, allowing it to interact with various transmembrane proteins like claudins, JAM-A, and occludin, as well as cytoplasmic proteins such as cortactin and α-catenin. ZO-1 plays a vital role in organizing the cytoskeleton, particularly in endothelial cells, where it controls barrier function, cell migration, and angiogenesis. It also participates in signaling pathways, such as the Hippo-independent YAP/TAZ pathway, influencing cell proliferation and transcriptional regulation. Additionally, ZO-1 interacts with YB-1 to regulate stress granule formation in endothelial cells during angiogenesis, providing cytoprotection against stress.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human stomach. Anti-ZO-1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-ZO-1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-ZO-1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-ZO-1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
