Product Specification
| Host |
Rat |
| Antigen |
CD366 (TIM-3) |
| Synonyms |
Hepatitis A virus cellular receptor 2 homolog; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3 (TIMD-3); T-cell immunoglobulin mucin receptor 3 (TIM-3); T-cell membrane protein 3; Timd3 |
| Location |
Membrane |
| Accession |
Q8VIM0 |
| Clone Number |
S-R478 |
| Antibody Type |
Rat mAb |
| Isotype |
Rat IgG2a,k |
| Application |
FCM |
| Reactivity |
Ms |
| Positive Sample |
C57BL/6 mouse splenocytes |
| Purification |
Protein G |
| Concentration |
0.2mg/ml |
| Conjugation |
Alexa Fluor® 488 |
| Physical Appearance |
Liquid |
| Storage Buffer |
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage |
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application |
dilution |
species |
| FCM |
5 μl per million cells in 100μl volume |
Ms |
Background
TIM-3 (T cell immunoglobulin and mucin domain-containing protein 3), also known as HAVCR2, is a crucial immune checkpoint molecule that is extensively expressed on various immune cells, including Th1, Th17, Treg, dendritic cells, monocytes, macrophages, and NK cells. The role of TIM-3 in the tumor immune microenvironment is particularly significant, as it is highly expressed on tumor-infiltrating CD8+ T cells and regulatory T cells (Tregs), and is co-expressed with PD-1, participating in the regulation of tumor immune evasion and T cell exhaustion. TIM-3 exerts its immune suppressive function by binding to its ligands, which include galectin-9 (Gal-9), phosphatidylserine (PtdSer), carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1), and high mobility group box 1 (HMGB1). These interactions lead to Th1 cell apoptosis, T cell exhaustion, and immune suppression, thereby facilitating tumor cells to evade immune surveillance. In cancer treatment strategies, TIM-3 has emerged as a promising target. Furthermore, the expression level of TIM-3 correlates with poor prognosis in certain types of cancer, making it a potential prognostic biomarker.
