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Alexa Fluor® 488 Mouse Anti-Human CD9 Antibody (S-632-33)

Alexa Fluor® 488 Mouse Anti-Human CD9 Antibody (S-632-33)

Catalog Number: S0B0171 Application: FCM Reactivity: Human Conjugation: Alexa Fluor 488 Brand: Starter
Price:
Regular price $110 USD
Regular price Sale price $110 USD
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Product Details

Product Specification


Host Mouse
Antigen CD9
Synonyms CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, MRP-1, Tetraspanin-29 (Tspan-29), p24, MIC3, TSPAN29
Immunogen Recombinant Protein
Location Cell membrane
Accession P21926
Clone Number S-632-33
Antibody Type Mouse mAb
Isotype IgG1,k
Application FCM
Reactivity Hu
Purification Protein G
Concentration 0.2mg/ml
Conjugation Alexa Fluor® 488
Physical Appearance Liquid
Storage Buffer PBS, 0.1% BSA, 0.01% Proclin 300
Stability & Storage 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.

Dilution


application dilution species
FCM 5 μl per million cells in 100μl volume

Background

CD9 belongs to the cell surface glycoprotein cross -membrane four -protein family, with four cross -membrane domains, a shorter outer domain (ECL1), and a longer extracellular domain (ECL2). It is expressed in a variety of hematopoietic cells and epithelial cells. For example, Pre B cells, B cell subset, activated T cells, basophils, eosinophils, macrophages, megakaryocytes, plasma cells, plasma cell precursors in germinal centers, and platelets. Broadcasting a series of cell processes such as cell adhesion, movement, membrane tissue and signal transfusion. The lowering of CD9 expression is related to the adverse prognosis and progress of various cancers. It is a favorable marker of gallbladder cancer, gastic GIST, malignant cortex and oral squamous cell carcinoma.

Picture

FC

Flow cytometric analysis of Human CD9 expression on HCT116. Cells from the HCT116 (Human colorectal carcinoma epithelial cell, Right) or K562 (Human chronic myelogenous leukemia lymphoblast, Left) was stained with Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (Black line histogram) and SDT Alexa Fluor® 488 Mouse Anti-Human CD9 Antibody (Red line histogram) at 1 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

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