AAK1 titration in ADP-Glo assay
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | AAK1,AP2-associated protein kinase 1 |
| Accession | Q2M2I8-1 |
| Amino Acid Sequence |
T27-A365 TSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPAKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIA |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 40.2 kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | 20mM Tris, 300mM Nacl, 1mM DTT, pH8.0 |
| Stability & Storage | -80℃ |
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Picture
Bioactivity


The AAK1 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the AAK1 protein , ATP and substrate at 25℃ for 120 min, then adding ADP-Glo reagent and reading RLU with BMG.

SEC-HPLC

