AAK1 titration in ADP-Glo assay
Product Details
Product Details
Product Specification
Species | Human |
Synonyms | AAK1,AP2-associated protein kinase 1 |
Accession | Q2M2I8-1 |
Amino Acid Sequence |
T27-A365 TSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPAKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIA |
Expression System | Baculovirus-InsectCells |
Molecular Weight | 40.2 kDa |
Purity | >95% by SDS-PAGE |
Endotoxin | <1EU/μg |
Conjugation | Unconjugated |
Physical Appearance | Liquid |
Storage Buffer | 20mM Tris, 300mM Nacl, 1mM DTT, pH8.0 |
Stability & Storage | -80℃ |
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Picture
Bioactivity


The AAK1 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the AAK1 protein , ATP and substrate at 25℃ for 120 min, then adding ADP-Glo reagent and reading RLU with BMG.

SEC-HPLC




