WB result of 14-3-3 sigma Recombinant Rabbit mAb
Primary antibody: 14-3-3 sigma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 28 kDa
14-3-3 sigma Recombinant Rabbit mAb (S-2017-59)
14-3-3 sigma Recombinant Rabbit mAb (S-2017-59)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | 14-3-3 sigma |
| Synonyms | 14-3-3 protein sigma; Epithelial cell marker protein 1; Stratifin; HME1; SFN |
| Immunogen | Synthetic Peptide |
| Location | Secreted, Cytoplasm, Nucleus |
| Accession | P31947 |
| Clone Number | S-2017-59 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms |
| Positive Sample | HeLa, A431, MCF7, Mouse colon |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms |
| IHC-P | 1:2000 | Hu |
| ICC | 1:500 | Hu |
Background
The 14-3-3 sigma protein, also known as stratifin, is a member of the 14-3-3 protein family that plays a crucial role in cell cycle regulation. It functions primarily to inhibit the progression of cells from the G2 phase to the M phase by preventing the activation of cyclin-dependent kinases. This protein is highly expressed in various tissues and is involved in maintaining genomic stability. Additionally, 14-3-3 sigma has been implicated in several cellular processes, including apoptosis and signal transduction pathways. Dysregulation of 14-3-3 sigma has been associated with certain cancers and other diseases, making it a potential target for therapeutic interventions.
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Picture
Western Blot
WB result of 14-3-3 sigma Recombinant Rabbit mAb
Primary antibody: 14-3-3 sigma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse colon lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 28 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-14-3-3 sigma antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-14-3-3 sigma antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-14-3-3 sigma antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human transitional cell carcinoma. Anti-14-3-3 sigma antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-14-3-3 sigma antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
