UniOne® TR-FRET Human IL6/IL6R Binding Kit

The assay kit employs Homogeneous Time-Resolved Fluorescence (TR-FRET) technology to measure the interaction between IL-6 and IL-6R. This method enables simple, rapid, and high-throughput identification of inhibitors and antibody-based blockers. As illustrated, the interaction between IL-6 and IL-6R is detected using a Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Acceptor-labeled anti-Tag2 antibody (TR-FRET acceptor). Upon binding of IL-6 to IL-6R, the donor and acceptor antibodies are brought into close proximity. Excitation of the donor antibody then triggers Fluorescence Resonance Energy Transfer (FRET) to the acceptor antibody, resulting in specific emission of a 665 nm wavelength signal from the acceptor. The intensity of this specific signal is proportional to the extent of IL-6/IL-6R interaction. This homogeneous assay features a straightforward workflow with no washing steps required.

Table 1. Reagents and Storage Conditions

* Avoid repeated freeze-thaw cycles of reagents. Aliquot upon first th