Standard Curve
Example of Monkey IL-6 standard curve in Assay Diluent HB9. Plotted are the background-subtracted data.
This standard curve is provided for demonstration only.
Monkey IL-6 OneStep ELISA Kit
Monkey IL-6 OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Mk |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 3.4%； Inter-assay: 5.8 |
| Sample type | Serum; EDTA Plasma, Heparin Plasma, Citrate Plasma; Cell Culture Supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.55 pg/mL |
| Range | 2.34 pg/mL – 150 pg/mL |
| Recovery | Serum: 90% EDTA Plasma: 90% Heparin Plasma:106% Citrate plasma: 107% Cell Culture Supernatant: 109% |
| Assay time | 60 minutes |
| Species reactivity | Mk |
| CROSS REACTIVITY | Hu |
Background
Monkey interleukin-6 (IL-6) is a pleiotropic, variably glycosylated cytokine that plays critical roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. The mature form of cynomolgus monkey IL-6 shares approximately 96% amino acid sequence identity with human IL-6, underscoring its high structural conservation and relevance as a translational model. The amino acid sequence of mature Rhesus Macaque IL-6 is approximately 186 amino acids (~21.1 kDa). It signals through a receptor complex composed of IL-6Rα and gp130 to regulate diverse biological functions, including B-cell differentiation, acute-phase protein production, and the modulation of inflammatory and immune responses. In non-human primate models, it is an important biomarker for studying inflammation, social influences on health, and preclinical drug development.
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ELISA
Spike Recovery
The recovery of Monkey IL-6 was evaluated in activated samples spiked with concentrations spanning the entire assay range.
Spike-and-dilution Linearity
To evaluate assay linearity, three samples were spiked with high levels of Monkey IL-6 in different matrices and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays).
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.
Determination of Minimum Detectable Dose (MDD)
The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
MDD = 2 × STDEV + AVERAGE
Cross-reactivity
The cross-reactivity between Monkey IL-6, Human IL-6, and Mouse IL-6 was assessed by testing both proteins in the same assay system. Serial dilutions of each protein were measured, and the dose-response curves were compared. No significant cross-reactivity was observed when Mouse IL-6 was tested in the Monkey IL-6 assay, as the signals remained within the background range of the assay.
Significant cross-reactivity was observed when Human IL-6 was tested in the Monkey IL-6 assay, as the signals remained within the background range of the assay.
HOOK Effect Threshold
The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.
Protocol Diagram
