Standard Curve
Example of Human MPO standard curve in Assay Diluent A1. Plotted are the background-subtracted data.
This standard curve is provided for demonstration only.
Human MPO OneStep ELISA Kit
Human MPO OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | Human MPO |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 3.3%； Inter-assay: 4.7% |
| Sample type | Serum; EDTA Plasma, Heparin Plasma, Citrate Plasma; Cell Culture Supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 5.13 pg/mL |
| Range | 23.44 pg/mL – 1500 pg/mL |
| Recovery | Serum: 93% EDTA Plasma: 99% Citrate plasma: 98% Heparin Plasma: 103% Cell Culture Supernatant: 84% |
| Assay time | 60 minutes |
| Species reactivity | Hu |
| CROSS REACTIVITY | Monkey, Hamster, Rat, Cat |
Background
Myeloperoxidase (MPO) is a heme-containing peroxidase enzyme predominantly expressed in neutrophil granulocytes, where it constitutes approximately 5% of the cell's dry weight, and plays an essential role in innate immunity by catalyzing the conversion of hydrogen peroxide and chloride ions into hypochlorous acid—a potent antimicrobial agent. Structurally, the mature MPO is a tetramer composed of two light and two heavy chains, with a covalently bound heme cofactor critical for its catalytic activity. While MPO is vital for host defense against pathogens, its oxidative products can also damage host tissues, linking the enzyme to various inflammatory and non-infectious diseases. Clinically, MPO serves as a valuable biomarker for cardiovascular disease, where elevated levels predict coronary artery disease, vulnerable atherosclerotic plaques, and higher mortality in acute coronary syndrome patients; it also shows promise in emergency triage for chest pain. Additionally, MPO has been implicated in Alzheimer's disease, multiple sclerosis, lung cancer, and MPO deficiency—a genetic immune disorder. Collectively, MPO is a multifaceted protein with critical roles in host defense and disease pathogenesis, and its applications span biomarker discovery, cancer prognosis, hematological diagnostics, research tools, and therapeutic development.
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ELISA
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays).
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.
Determination of Minimum Detectable Dose (MDD)
The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
MDD = 2 × STDEV + AVERAGE
Cross-reactivity
The cross-reactivity between Human MPO and Mouse MPO was assessed by testing both proteins in the same assay system. Serial dilutions of each protein were measured, and the dose-response curves were compared. No significant cross-reactivity was observed when Mouse MPO was tested in the Human MPO assay, as the signals remained within the background range of the assay.
HOOK Effect Threshold
The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.
Protocol Diagram
