Linearity
The concentrations of IFN-γ were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: BALB/c plasma and C57BL/6 serum(100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 111.135 pg/mL in C57BL/6 serum.
UltraSensKine Mouse IFN-γ ELISA Kit
UltraSensKine Mouse IFN-γ ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IFN-γ |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 3.7%； Inter-assay: 5.5% |
| Sample type | Cell culture supernatant |
| Assay type | Sandwich(quantitative) |
| Sensitivity | 1.29pg/mL |
| Range | 7.81pg/mL-500pg/mL |
| Recovery | Cell culture supernatant: 103% |
| Assay time | 2.5 hours |
| Species reactivity | Mouse |
Background
Mouse IFN-γ, or Interferon-gamma, is a crucial cytokine primarily produced by natural killer (NK) cells and T lymphocytes, particularly Th1 cells and cytotoxic T cells. It plays a pivotal role in the immune response, acting as a key mediator in both innate and adaptive immunity. IFN-γ is known for its potent antiviral, immunoregulatory, and anti-tumor properties. It enhances the presentation of antigens by upregulating major histocompatibility complex (MHC) class I and II molecules on antigen-presenting cells, thereby facilitating a more robust immune response. Additionally, IFN-γ activates macrophages, increasing their ability to phagocytose and destroy pathogens. It also promotes the differentiation of naive T cells into Th1 cells, further amplifying the immune response. In research, mouse IFN-γ is widely used to study immune responses, inflammatory diseases, and host-pathogen interactions, providing valuable insights into the mechanisms of immune regulation and potential therapeutic strategies.
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