WB result of Tyrosine Hydroxylase Recombinant Rabbit mAb
Primary antibody: Tyrosine Hydroxylase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 60 kDa
Tyrosine Hydroxylase Recombinant Rabbit mAb (S-1001-26)
Tyrosine Hydroxylase Recombinant Rabbit mAb (S-1001-26)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Tyrosine Hydroxylase |
| Synonyms | Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase (TH), TH, TYH |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P07101 |
| Clone Number | S-1001-26 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC-P | 1:250 | |
| ICC | 1:50 |
Background
Tyrosine hydroxylase (TH) is a pivotal enzyme in the biosynthesis of catecholamines, such as dopamine, norepinephrine, and epinephrine. It catalyzes the conversion of the amino acid tyrosine to 3,4-dihydroxyphenylalanine (L-DOPA), which is the first and rate-limiting step in this biosynthetic pathway. This conversion is dependent on the cofactor (6R)-L-erythro-tetrahydrobiopterin (BH4) and ferrous iron, with the latter being directly bound to TH and oxidized to Fe3+ by O2. TH is subject to intricate regulation at both transcriptional and post-translational levels. Rapid regulation can occur through end-product inhibition influenced by the phosphorylation status of N-terminal serines, modulating the enzyme's sensitivity to the local concentration of catecholamines. Phosphorylation at specific serine residues, such as Ser40 and Ser31, can enhance TH activity and catecholamine synthesis.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human adrenal gland. Anti-Tyrosine Hydroxylase antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in PC-12 cells. Anti- Tyrosine Hydroxylase antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
