WB result of α/β-Tubulin Rabbit pAb
Primary antibody: α/β-Tubulin Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
α/β-Tubulin Antibody Rabbit Polyclonal Antibody
α/β-Tubulin Antibody Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | α/β-Tubulin |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P07437、P68363 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Ms, Rt, Mk |
| Positive Sample | NIH/3T3, mouse brain, C6, rat brain, COS-7 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Ms, Rt, Mk |
| IHC-P | 1:200 | Ms, Rt |
| ICC | 1:500 | Hu |
Background
The α/β-Tubulin heterodimer is the fundamental building block of microtubules. These proteins polymerize in a GTP-dependent manner to form the polarized protofilaments of the microtubule lattice. α-Tubulin typically binds GTP irreversibly, providing structural stability, while the hydrolysis of GTP to GDP on β-Tubulin is the key driver of microtubule dynamic instability—the stochastic switch between phases of growth and rapid disassembly. Both subunits share a highly conserved GTPase domain and interact longitudinally to extend the hollow cylindrical structure. They are essential for critical cellular processes including cell division, ciliary motility, and intracellular transport. The functionality of microtubules is precisely regulated by tubulin expression levels and post-translational modifications, such as acetylation and tyrosination. Consequently, α/β-tubulin is a major target for cancer chemotherapy (e.g., microtubule-targeting agents) and a focal point in neurodegenerative disease research.
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Western Blot
WB result of α/β-Tubulin Rabbit pAb
Primary antibody: α/β-Tubulin Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
WB result of α/β-Tubulin Rabbit pAb
Primary antibody: α/β-Tubulin Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- α/β-Tubulin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti- α/β-Tubulin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- α/β-Tubulin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti- α/β-Tubulin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- α/β-Tubulin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
