IHC shows positive staining in paraffin-embedded human synovial sarcoma. Anti- SS18-SSX antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
SS18-SSX Recombinant Rabbit mAb, PBS Only (SDT-4202)
SS18-SSX Recombinant Rabbit mAb, PBS Only (SDT-4202)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SS18-SSX |
| Synonyms | SYT-SSX1; SS18/SSX1 fusion; SSX1/SSXT fusion |
| Location | Nucleus |
| Accession | A4PIV8 |
| Clone Number | SDT-4202 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS |
| Stability & Storage | 12 months from date of receipt / reconstitution, 4°C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:500 | Hu |
Background
The SS18-SSX fusion protein is the defining oncogenic driver of synovial sarcoma, arising from the chromosomal translocation t(X;18)(p11;q11) that fuses the SS18 gene on chromosome 18 with SSX1, SSX2, or rarely SSX4 on the X chromosome, producing a chimeric protein in which the C-terminal 8 amino acids of SS18 are replaced by 78 residues from SSX; this fusion retains the N-terminal SNH domain of SS18 that mediates incorporation into SWI/SNF (BAF) chromatin-remodeling complexes, but the SSX moiety recruits polycomb repressive complexes (especially PRC1.1 via KDM2B) and binds H2AK119ub-rich regions, thereby hijacking BAF targeting to aberrantly activate developmentally repressed genes, evict the tumor-suppressive BAF47 (SMARCB1/INI1) subunit, and globally reprogram the epigenetic landscape to enforce malignant transformation.
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Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti- SS18-SSX antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung squamous cell carcinoma. Anti- SS18-SSX antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
