WB result of Sialoadhesin/CD169 Recombinant Rabbit mAb
Primary antibody: Sialoadhesin/CD169 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated THP-1 whole cell lysate 20 µg
Lane 2: THP-1 treated with PMA (100 nM, 24 hours), LPS (10 ng/ml, 7 hours）and BFA (5 µg/ml, 5 hours) whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 183 kDa
Observed MW: 270 kDa
Sialoadhesin/CD169 Recombinant Rabbit mAb, PBS Only (SDT-3228-8)
Sialoadhesin/CD169 Recombinant Rabbit mAb, PBS Only (SDT-3228-8)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD169 |
| Synonyms | Sialoadhesin; Sheep erythrocyte receptor (SER); Sialic acid-binding Ig-like lectin 1 (Siglec-1); Sa; Sn; Siglec1 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | Q62230 |
| Clone Number | SDT-3228-8 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | PMA, LPS and BFA treated THP-1 cells, LPS treated RAW264.7, mouse spleen, mouse liver, rat spleen, rat liver |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS |
| Stability & Storage | 12 months from date of receipt / reconstitution, 4 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Ms |
Background
CD169, also known as Siglec-1 or sialoadhesin, is a type I transmembrane glycoprotein of the immunoglobulin superfamily expressed predominantly on subsets of tissue-resident macrophages and some dendritic cells; it contains 17 extracellular Ig-like domains that together project ~33 nm from the plasma membrane to enable high-avidity binding to α2,3-linked sialic acid moieties on glycoproteins and glycolipids of host cells or pathogens, thereby facilitating pathogen capture, antigen presentation, and adaptive immune priming while simultaneously serving as an entry portal for viruses such as HIV-1, PRRSV, and SARS-CoV-2, and its interferon-inducible expression correlates with both protective immunity and disease severity in infections, cancer, and chronic inflammatory disorders.
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Picture
Western Blot
WB result of Sialoadhesin/CD169 Recombinant Rabbit mAb
Primary antibody: Sialoadhesin/CD169 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated RAW264.7 whole cell lysate 20 µg
Lane 2: RAW264.7 treated with 1 µg/ml LPS for 24 hours whole cell lysate 20 µg
Lane 3: mouse kidney lysate 20 µg
Lane 4: mouse spleen lysate 20 µg
Lane 5: mouse liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 183 kDa
Observed MW: 270 kDa
WB result of Sialoadhesin/CD169 Recombinant Rabbit mAb
Primary antibody: Sialoadhesin/CD169 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Lane 3: rat liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 183 kDa
Observed MW: 270 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat cerebral cortex. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC analysis of Raw264.7 cells treated with LPS(1ug/ml,24h) (top panel) and untreated Raw264.7 cells (below panel). Anti- Sialoadhesin/CD169 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
