S-RMab® PD-L1 Recombinant Rabbit mAb (SDT-119-38)

Catalog Number: S0B2067 Application: IHC-P Reactivity: Human Conjugation: Unconjugated Brand: Starter

Price:

$120.00 USD

Size: 25μl 100μl 500μl

Quantity:

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Product Details

Product Specification

Host	Rabbit
Antigen	PD-L1
Synonyms	CD274, B7-H1
Immunogen	Recombinant Protein
Location	Membrane
Accession	Q9NZQ7
Clone Number	SDT-119-38
Antibody Type	Rabbit mAb
Application	IHC-P
Reactivity	Hu
Purification	Protein A
Concentration	0.5 mg/ml
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
IHC-P	1:100	null

Background

Programmed death-ligand 1 (PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a protein that in humans is encoded by the CD274 gene. Programmed death-ligand 1 (PD-L1) is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the adaptive arm of immune systems during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. The binding of PD-L1 to the inhibitory checkpoint molecule PD-1 transmits an inhibitory signal based on interaction with phosphatases (SHP-1 or SHP-2) via Immunoreceptor Tyrosine-Based Switch Motif (ITSM). This reduces the proliferation of antigen-specific T-cells in lymph nodes, while simultaneously reducing apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells) - further mediated by a lower regulation of the gene Bcl-2. Upregulation of PD-L1 on immune cells (especially myeloid cells) can also lead to formation of an immunosuppressive environment in a highly localized manner that also allow the cancer cells to proliferate.

Picture

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human tonsil.

Anti-PD-L1 antibody was used at 1/100 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.