WB result of Phospho-p38 MAPK (Thr180/Tyr182) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-p38 MAPK (Thr180/Tyr182) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated Jurkat whole cell lysate 20 µg
Lane 2: Jurkat treated with 25 µg/ml anisomycin for 20 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 38 kDa
Phospho-p38 MAPK (Thr180/Tyr182) Recombinant Rabbit mAb (S-3861)
Phospho-p38 MAPK (Thr180/Tyr182) Recombinant Rabbit mAb (S-3861)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-p38 MAPK (Thr180/Tyr182) |
| Synonyms | Mitogen-activated protein kinase 14, MAP kinase 14, MAPK14, CSAID-binding protein, CSBP, MAP kinase MXI2, MAX-interacting protein 2, MAP kinase p38 alpha, SAPK2a |
| Location | Cytoplasm, Nucleus |
| Accession | Q16539 |
| Clone Number | S-3861 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | anisomycin treated Jurkat, anisomycin treated NIH/3T3, anisomycin treated C6, anisomycin treated COS-7 |
| Predicted Reactivity | Dm, Pg, Sc |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| ICC | 1:500 | Hu |
Background
Phospho-p38 MAPK (Thr180/Tyr182) refers to the active form of the p38 mitogen-activated protein kinase that is dually phosphorylated at threonine 180 (Thr180) and tyrosine 182 (Tyr182) within its activation loop. As a central component of the cellular stress response signaling pathway, p38 MAPK is often termed the "stress-activated protein kinase." When cells are exposed to external stressors such as oxidative stress, osmotic shock, UV radiation, or inflammatory cytokines, upstream kinases (e.g., MKK3/MKK6) specifically recognize and phosphorylate these two residues. This dual phosphorylation induces a conformational change in the p38 protein, leading to its full activation. The activated Phospho-p38 MAPK then translocates from the cytoplasm to the nucleus, where it phosphorylates a series of downstream transcription factors (e.g., ATF-2, CHOP, MEF2) and other kinases, ultimately regulating critical biological processes including inflammatory responses, apoptosis, differentiation, autophagy, and cell cycle arrest. Consequently, detecting the level of Phospho-p38 MAPK (Thr180/Tyr182) serves as the most direct and crucial molecular indicator for assessing the activation status of the p38 MAPK pathway within cells, and it is widely utilized in fields such as immunology, neuroscience, cancer research, and drug development.
Picture
Picture
Western Blot
WB result of Phospho-p38 MAPK (Thr180/Tyr182) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-p38 MAPK (Thr180/Tyr182) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 25 µg/ml anisomycin for 20 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 38 kDa
WB result of Phospho-p38 MAPK (Thr180/Tyr182) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-p38 MAPK (Thr180/Tyr182) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with UV (50 mJ/cm2, 30 minutes recovery) whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 38 kDa
WB result of Phospho-p38 MAPK (Thr180/Tyr182) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-p38 MAPK (Thr180/Tyr182) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated COS-7 whole cell lysate 20 µg
Lane 2: COS-7 treated with UV (50 mJ/cm2, 30 minutes recovery) whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 38 kDa
Immunocytochemistry
ICC analysis of HeLa cells treated with anisomycin(25ug/ml,20mins) (top panel) and untreated HeLa cells (below panel). Anti- Phospho-p38 MAPK (Thr180/Tyr182) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
