WB result of Periostin Recombinant Rabbit mAb
Primary antibody: Periostin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SW620 whole cell lysate 20 µg
Lane 2: NCI-H69 whole cell lysate 20 µg
Negative control: SW620 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 93 kDa
Observed MW: 90 kDa
Periostin Recombinant Rabbit mAb (S-2824-17)
Periostin Recombinant Rabbit mAb (S-2824-17)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Periostin |
| Synonyms | PN; Osteoblast-specific factor 2 (OSF-2); OSF2; POSTN |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | Q15063 |
| Clone Number | S-2824-17 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | NCI-H69 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:2000 | Hu |
Background
Periostin is a 90-kDa secreted matricellular protein that consists of a typical signal peptide, an EMI domain, four tandem fasciclin-1 (FAS-1) domains implicated in cell adhesion and protein–protein interactions, and a C-terminal splice variant-rich domain that can generate six isoforms; it is predominantly expressed in collagen-rich connective tissues such as periodontal ligament, periosteum, heart valves, skin, and fibrotic lesions, where it is rapidly induced by TGF-β, IL-4/IL-13, mechanical stress or hypoxia and then deposited into the extracellular matrix via integrin-mediated binding to αvβ3, αvβ5 or α6β4 receptors on fibroblasts, thereby promoting collagen fibrillogenesis, cross-linking and tissue remodeling during embryonic development, wound healing, bone fracture repair, cardiac remodeling after myocardial infarction, and pathological fibrosis in asthma, atopic dermatitis, liver cirrhosis and various solid tumors, while also activating PI3K/Akt, FAK/Src, ERK and YAP/TAZ signaling pathways that enhance epithelial–mesenchymal transition, angiogenesis, cancer cell invasion, metastatic niche formation and chemoresistance, making it a valuable biomarker for disease progression and a potential therapeutic target for fibrosis and cancer.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-Periostin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Periostin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Periostin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Periostin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-Periostin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
