WB result of p44/42 MAPK (Erk1/2) Recombinant Rabbit mAb
Primary antibody: p44/42 MAPK (Erk1/2) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43, 41 kDa
Observed MW: 44, 42 kDa
p44/42 MAPK (Erk1/2) Recombinant Rabbit mAb (S-3600)
p44/42 MAPK (Erk1/2) Recombinant Rabbit mAb (S-3600)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Erk1/2 |
| Synonyms | Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2 (ERK-2); MAP kinase isoform p42 (p42-MAPK); Mitogen-activated protein kinase 2 (MAP kinase 2; MAPK 2); ERK2; PRKM1; PRKM2; MAPK1 |
| Location | Cytoplasm, Cytoskeleton, Nucleus |
| Accession | P28482、P27361 |
| Clone Number | S-3600 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, ICFCM |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | MCF7, HepG2, A431, HeLa, NIH/3T3, PC-12, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:10000 | Hu, Ms, Rt, Mk |
| ICC | 1:500 | Hu |
| ICFCM | 1:500 | Hu |
Background
Erk1/2 proteins, also known as extracellular signal-regulated kinases 1 and 2, are key components of the MAPK (mitogen-activated protein kinase) signaling pathway. They play roles crucial in regulating various cellular processes such as cell proliferation, differentiation, survival, and apoptosis. Erk1/2 can be activated by a wide range of extracellular stimuli including growth factors, cytokines, and hormones. Once activated, they phosphorylate a cascade of downstream targets, thereby transmitting signals from the cell surface to the nucleus and modulating gene expression. Dysregulation of Erk1/2 signaling has been implicated in numerous diseases, including cancer andde neurogenerative disorders, making them important targets for therapeutic intervention.
Picture
Picture
Western Blot
WB result of p44/42 MAPK (Erk1/2) Recombinant Rabbit mAb
Primary antibody: p44/42 MAPK (Erk1/2) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43, 41 kDa
Observed MW: 44, 42 kDa
WB result of p44/42 MAPK (Erk1/2) Recombinant Rabbit mAb
Primary antibody: p44/42 MAPK (Erk1/2) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43, 41 kDa
Observed MW: 44, 42 kDa
WB result of p44/42 MAPK (Erk1/2) Recombinant Rabbit mAb
Primary antibody: p44/42 MAPK (Erk1/2) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43, 41 kDa
Observed MW: 44, 42 kDa
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell) labelling p44/42 MAPK (Erk1/2) antibody at 1/500 dilution (0.1 μg)/ (red) compared with a Rabbit monoclonal IgG (black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunocytochemistry
ICC shows positive staining in A431 cells. Anti- p44/42 MAPK (Erk1/2) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
