WB result of NKX3.1 Recombinant Rabbit mAb
Primary antibody: NKX3.1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: LNCaP whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: DU 145 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate; PC-3 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 26 kDa
Observed MW: 30 kDa
NKX3.1 Recombinant Rabbit mAb,PBS Only(S-2778-63)
NKX3.1 Recombinant Rabbit mAb,PBS Only(S-2778-63)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NKX3.1 |
| Synonyms | Homeobox protein Nkx-3.1; Homeobox protein NK-3 homolog A; NKX3A; NKX3-1 |
| Immunogen | Recombinant Protein |
| Location | Nucleus |
| Accession | Q99801 |
| Clone Number | S-2778-63 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | LNCaP, Jurkat, DU 145 |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS |
| Stability & Storage | 12 months from date of receipt / reconstitution, 4°C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:200 | Hu |
| ICC | 1:500 | Hu |
Background
NKX3.1 is a prostate-specific homeodomain transcription factor that acts as a dose-dependent tumor suppressor by integrating developmental and metabolic cues: in the nucleus it partners with the androgen receptor, FoxA1 and serum response factor to coregulate differentiation genes and a “protein-trafficking” network that includes RAB3B, while in mitochondria it is imported via HSPA9 in response to oxidative stress, binds mtDNA and up-regulates electron-transport-chain genes to restore oxidative phosphorylation, thereby limiting ROS-induced DNA damage; haploinsufficiency, allelic loss or cancer-associated point mutations (e.g., T164A) disrupt these functions, leading to prostatic intraepithelial neoplasia, progression to castration-resistant disease, and reduced survival, whereas high mitochondrial NKX3.1 predicts favorable prognosis, and secreted NKX3.1 can even act as a para-transcription factor to inhibit growth of neighboring cells, illustrating its multifaceted role in prostate development, metabolic homeostasis and cancer suppression.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human prostate. Anti-NKX3.1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-NKX3.1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-NKX3.1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer (case 1). Anti-NKX3.1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer (case 2). Anti-NKX3.1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung squamous cell carcinoma. Anti-NKX3.1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in LNCaP cells (top panel) and negative staining in PC-3 cells (below panel). Anti-NKX3.1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
