WB result of MUC4 Rabbit mAb
Primary antibody: MUC4 Rabbit mAb at 1/1000 dilution
Lane 1: PANC-1 whole cell lysate 20 µg
Lane 2: BxPC-3 whole cell lysate 20 µg
Negative control: PANC-1 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 232 kDa
Observed MW: 110~200 kDa
MUC4 Recombinant Rabbit mAb,PBS Only (SDT-841-13)
MUC4 Recombinant Rabbit mAb,PBS Only (SDT-841-13)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MUC4 |
| Synonyms | Ascites sialoglycoprotein (ASGP), Pancreatic adenocarcinoma mucin, Testis mucin, Tracheobronchial mucin, Mucin4, MUC-4 |
| Immunogen | Recombinant Protein |
| Location | Secreted, Cell membrane |
| Accession | Q99102 |
| Clone Number | SDT-841-13 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS |
| Stability & Storage | 12 months from date of receipt / reconstitution, 4°C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC-P | 1:1000 | |
| IP | 1:50 |
Background
Mucin 4 (MUC4) is a highly glycosylated type I transmembrane glycoprotein. Normally acts as barrier to apical surface of epithelial cells, playing a protective and regulatory role. MUC4 expression is erroneous in many carcinomas and sarcomas, including sclerosing epithelioid fibrosarcoma and pancreatic adenocarcinoma. Immunohistochemical staining for MUC4 has been discussed as a potential biomarker for detection of these cancers. MUC4 can serve as a ligand for the oncogenic receptor ErbB2 and a modulator of its phosphorylation and signaling. MUC4 is frequently aberrantly expressed in epithelial tumors and can promote tumor growth and metastasis.
Picture
Picture
Western Blot
IP
MUC4 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating MUC4 in 0.4 mg BxPC-3 whole cell lysate.
Western blot was performed on the immunoprecipitate using MUC4 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: BxPC-3 whole cell lysate 20 µg (Input)
Lane 2: MUC4 Rabbit mAb IP in BxPC-3 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in BxPC-3 whole cell lysate
Predicted MW: 232 kDa
Observed MW: 110~200 kDa
（This blot was developed with high sensitivity substrate）
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-MUC4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung. Anti-MUC4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-MUC4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-MUC4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human mesothelioma. Anti-MUC4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human leiomyosarcoma. Anti-MUC4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
