Standard curve
Example of Mouse IgM standard curve in Assay Diluent #1.
Mouse IgM OneStep ELISA Kit
Mouse IgM OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IgM |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision |
Intra-assay: 3.2%； Inter-assay: 6.8% |
| Sample type | Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.298 ng/ml |
| Range | 1.25 ng/mL - 80 ng/mL |
| Recovery | Serum 92% Plasma 102% |
| Assay time | 60 minutes |
| Species reactivity | Mouse |
| CROSS REACTIVITY | / |
| INTERFERENCE | / |
| NIBSC convert | / |
Background
Mouse immunoglobulin M (IgM) is a crucial component of the humoral immune system and is the first antibody produced by B-cells in response to an antigenic challenge. It exists in two forms: a pentameric (19S) form found in serum and a monomeric (8S) form on the surface of lymphocytes. The IgM pentamer, which is the predominant form in circulation, consists of five monomeric units and has a molecular weight of approximately 900 kDa, while the hexameric form has a molecular weight of about 1050 kDa. Each monomer of IgM is composed of two heavy chains and two light chains, with the heavy chain having four constant domains (CH1-CH4) and the light chain having one constant domain (CL). The CH4 domain facilitates oligomerization, leading to the formation of penta- or hexamers, and these oligomers are covalently linked via disulfide bonds, resembling a snowflake structure. A joining (J) chain, a 15 kDa polypeptide, is essential for the secretion of IgM into mucosal areas.
Picture
Picture
ELISA
Linearity
The concentrations of Mouse IgM were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Mouse serum was 4×10^3 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 262.88 ug/mL in Mouse serum.
#2 sample is undiluted samples are as follows: Mouse plasma was 4×10^3 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 335.85 ug/mL in Mouse plasma.
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