WB result of Islet1 Recombinant Rabbit mAb
Primary antibody: Islet1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 40 kDa
Islet1 Recombinant Rabbit mAb,PBS Only(SDT-2349-17)
Islet1 Recombinant Rabbit mAb,PBS Only(SDT-2349-17)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Islet1 |
| Synonyms | Insulin gene enhancer protein ISL-1; Islet-1; ISL1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P61371 |
| Clone Number | SDT-2349-17 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | SH-SY5Y, mouse pancreas |
| Predicted Reactivity | Zf, Ck |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 4 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms |
| IHC-P | 1:250 | Hu, Ms, Rt |
Background
Islet1 is a transcription factor protein that plays a crucial role in the development and differentiation of various cell types, particularly in the nervous system and the pancreas. In the nervous system, it is involved in the specification and maturation of motor neurons and interneurons, helping to guide their proper formation and function during embryonic development. In the pancreas, Islet1 is essential for the development of endocrine cells, including those that produce insulin and other hormones. Its expression is tightly regulated and its dysfunction has implicated been in developmental disorders and diseases such as diabetes.
Picture
Picture
Western Blot
WB result of Islet1 Recombinant Rabbit mAb
Primary antibody: Islet1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse pancreas lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 39 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-Islet1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreas. Anti-Islet1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-Islet1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human non-small cell lung cancer. Anti-Islet1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse pancreas. Anti-Islet1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat pancreas. Anti-Islet1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
