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Human RET Protein, His Tag

Human RET Protein, His Tag

Catalog Number: S0A2224 Brand: Starter
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$170 USD
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Product Details

Product Specification


Species Human
Synonyms Proto-oncogene tyrosine-protein kinase receptor Ret, Cadherin family member 12, Proto-oncogene c-Ret, CDHF12, CDHR16, PTC
Accession P07949
Amino Acid Sequence

Protein sequence (P07949, Leu29-Arg635, with C-His Tag) LYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQEDTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENRPPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAPTFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNRNLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCSTLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGRCEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLCDELCR
Expression System HEK293
Molecular Weight Predicted MW: 69.5 kDa Observed MW: 100-120 kDa
Purity ＞95% by SDS-PAGE
Endotoxin <1EU/μg
Conjugation Unconjugated
Physical Appearance Lyophilized powder
Storage Buffer

Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4 with 3% trehalose.
Reconstitution Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
Stability & Storage

12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.

Background

The RET is a receptor tyrosine kinase crucial for normal cell development and survival. It is activated by binding to glial cell line-derived neurotrophic factor (GDNF) family ligands, leading to dimerization and autophosphorylation. This triggers downstream signaling pathways like MAPK and PI3K-AKT, which regulate proliferation, differentiation, and migration, particularly in neural crest and genitourinary lineages. Gain-of-function mutations in RET cause constitutive activation, driving oncogenesis in medullary thyroid cancer and other malignancies. Conversely, loss-of-function mutations are associated with developmental disorders like Hirschsprung's disease. RET is thus a critical therapeutic target in both oncology and neurodevelopment.

Picture

SDS-PAGE

2μg（R: reducing conditions）