WB result of Histone H3 (tri methyl K27) Recombinant Rabbit mAb
Primary antibody: Histone H3 (tri methyl K27) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrate
Histone H3 (tri methyl K27) Recombinant Rabbit mAb (S-4098)
Histone H3 (tri methyl K27) Recombinant Rabbit mAb (S-4098)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Histone H3 (tri methyl K27) |
| Synonyms | H3K27me3 |
| Location | Nucleus |
| Accession | P68431 |
| Clone Number | S-4098 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, NIH/3T3 |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:100 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
Background
Histone H3 lysine 27 trimethylation (abbreviated as H3K27me3) is a classic transcription-repressive epigenetic mark, catalyzed by the core catalytic subunits EZH1 or EZH2 of Polycomb Repressive Complex 2 (PRC2). This modification serves as the central executor of PRC2-mediated gene silencing. Its primary function is to recruit and stabilize effector proteins, such as the PRC1 complex, collectively promoting the formation of compact, higher-order chromatin structures to stably and long-term suppress the expression of specific genes at the epigenetic level. H3K27me3 plays a crucial role in development, extensively participating in key biological processes including cell fate determination, maintenance of stem cell pluripotency, somatic cell reprogramming, and X-chromosome inactivation. By precisely establishing and maintaining a "gene expression blueprint," it ensures the correct identity of cells. However, when this powerful silencing function becomes dysregulated, it can lead to severe consequences. For instance, gain-of-function mutations or aberrant expression of genes encoding PRC2 components (such as EZH2) result in disrupted H3K27me3 levels, a phenomenon widely observed in various malignancies including lymphoma, prostate cancer, sarcoma, and glioma, making it a key driver of tumorigenesis. Notably, its function is often antagonistic to the activating mark H3K4me3, and together they can form "bivalent chromatin domains," which co-mark key developmental regulator genes in embryonic stem cells that are poised for activation upon differentiation. Therefore, H3K27me3 is not only a central molecule in developmental biology and disease research but has also become an important target for the development of current epigenetic drugs, such as EZH2 inhibitors.
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Picture
Western Blot
WB result of Histone H3 (tri methyl K27) Recombinant Rabbit mAb
Primary antibody: Histone H3 (tri methyl K27) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Histone H3 (tri methyl K27) antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Histone H3 (tri methyl K27) antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-Histone H3 (tri methyl K27) antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- Histone H3 (tri methyl K27) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- Histone H3 (tri methyl K27) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
