WB result of FOXA1 Recombinant Rabbit mAb
Primary antibody: FOXA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Daudi whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: PC-3 whole cell lysate 20 µg
Lane 4: LNCaP whole cell lysate 5 µg
Lane 5: MCF7 whole cell lysate 20 µg
Negative control: Daudi whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 50 kDa
FOXA1 Recombinant Rabbit mAb (SDT-2779-17)
FOXA1 Recombinant Rabbit mAb (SDT-2779-17)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FOXA1 |
| Synonyms | Hepatocyte nuclear factor 3-alpha; HNF-3-alpha; HNF-3A; Forkhead box protein A1; Transcription factor 3A (TCF-3A); HNF3A; TCF3A |
| Immunogen | Recombinant Protein |
| Location | Nucleus |
| Accession | P55317 |
| Clone Number | SDT-2779-17 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HepG2, PC-3, LNCaP, MCF7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:200 | Hu |
| ICC | 1:500 | Hu |
| IF | 1:100 | Hu |
Background
FOXA1, a 46-kDa, 472-aa winged-helix/forkhead DNA-binding pioneer transcription factor, opens compacted chromatin by partial nucleosome unfolding, thereby enabling recruitment of steroid-hormone receptors and lineage-specific transcriptional programs that govern development, differentiation, and adult homeostasis of liver, pancreas, lung, prostate, and breast; in cancer it acts as a context-dependent tumor suppressor or oncogene—most notably driving estrogen-receptor-α (ERα) binding and luminal breast/prostate identity—through genomic rearrangements, hotspot mutations (e.g., R219S/W), or altered protein–protein interactions that reprogram enhancer landscapes, control epithelial–mesenchymal plasticity, and modulate therapeutic response to endocrine agents, making FOXA1 a critical determinant of lineage fidelity, drug sensitivity, and patient prognosis.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-FOXA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-FOXA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-FOXA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-FOXA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-FOXA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-FOXA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-FOXA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in MCF7 cells (top panel) and negative staining in Daudi cells (below panel). Anti-FOXA1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Immunofluorescence
IF shows positive staining in paraffin-embedded human breast cancer. Anti- FOXA1 antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 647) (S0B4005) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human prostatic cancer. Anti- FOXA1 antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 647) (S0B4005) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
