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FAP Recombinant Rabbit mAb (S-3094-29)

FAP Recombinant Rabbit mAb (S-3094-29)

Catalog Number: S0B6526 Application: WB, IHC-P Reactivity: Human Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen FAP
Synonyms Prolyl endopeptidase FAP; 170 kDa melanoma membrane-bound gelatinase; Dipeptidyl peptidase FAP; Fibroblast activation protein alpha (FAPalpha); Gelatine degradation protease FAP; Integral membrane serine protease
Immunogen Synthetic Peptide
Location Cytoplasm, Secreted, Cell membrane
Accession Q12884
Clone Number S-3094-29
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Hu
Positive Sample SK-MEL-28, WI-38
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Hu
IHC-P 1:2000-1:4000 Hu

Background

Fibroblast Activation Protein (FAP) is a 97-kDa type-II transmembrane serine protease that is virtually absent from healthy adult tissues but becomes highly and persistently up-regulated on activated fibroblasts during wound healing, fibrosis, arthritis, atherosclerosis and in the stroma of over 90 % of epithelial cancers, where its unique endopeptidase (Gly-Pro-X) and dipeptidyl-peptidase activities drive extracellular-matrix remodelling, immune evasion, M2 macrophage polarization and tumour-cell proliferation, migration and invasion via signalling axes such as ITGB1/PI3K/AKT and interactions with fibronectin, collagen and immune-checkpoint ligands, making it a powerful prognostic biomarker and an attractive therapeutic target across multiple malignancies and fibrotic diseases.

Picture

Western Blot

WB result of FAP Recombinant Rabbit mAb
Primary antibody: FAP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: SK-MEL-28 whole cell lysate 20 µg
Lane 3: WI-38 whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 90 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human stomach. Anti-FAP antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-FAP antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-FAP antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-FAP antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-FAP antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.