IHC shows positive staining in paraffin-embedded human tonsil. Anti-CXCL13 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
CXCL13 Recombinant Rabbit mAb,PBS Only (SDT-2703-132)
CXCL13 Recombinant Rabbit mAb,PBS Only (SDT-2703-132)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CXCL13 |
| Synonyms | C-X-C motif chemokine 13; Angie; B cell-attracting chemokine 1 (BCA-1); B lymphocyte chemoattractant; CXC chemokine BLC; Small-inducible cytokine B13; BCA1; BLC; SCYB13 |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | O43927 |
| Clone Number | SDT-2703-132 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS |
| Stability & Storage | 12 months from date of receipt / reconstitution, 4 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:500 | Hu |
| IF | 1:100 | Hu |
Background
CXCL13, also called BCA-1 or BLC, is a homeostatic CXC chemokine that acts as the main chemoattractant for B lymphocytes and follicular helper T cells through exclusive binding to the G-protein-coupled receptor CXCR5, thereby guiding their migration into lymphoid follicles and organizing the microarchitecture of secondary lymphoid organs such as lymph nodes and spleen; secreted chiefly by follicular dendritic cells, stromal cells, and macrophages, it triggers downstream signaling cascades including PLC-β/PKCα, MAPK, PI3K-Akt, and NF-κB that promote chemotaxis, adhesion, and survival of B cells, and its dysregulation—manifested by elevated serum or tissue levels—has been mechanistically linked to autoimmune diseases (e.g., rheumatoid arthritis, Sjögren’s syndrome, systemic lupus erythematosus), chronic inflammatory disorders, B-cell malignancies, and neuroinflammatory conditions like multiple sclerosis, making it both a pathogenic driver and a clinically useful biomarker for disease activity and prognosis.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spleen. Anti-CXCL13 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-CXCL13 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CXCL13 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human tonsil. Anti-CXCL13 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
