Flow cytometric analysis of Human CD226 expression on HeLa cells. Cells from the HeLa (Human cervix adenocarcinoma epithelial cell, Right) or MOLT-4 (Human lymphoblastic leukemia T lymphoblast, Left) was stained with either Biotin Mouse IgG2b, κ Isotype Control (Black line histogram) or SDT Biotin Mouse Anti-Human CD226 antibody (Red line histogram) at 5 μl/test followed by Sav-iFluor 488, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Mouse Anti-Human CD266 (TWEAK Receptor) Antibody (S-3902)
Biotin Mouse Anti-Human CD266 (TWEAK Receptor) Antibody (S-3902)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD266 (TWEAK Receptor) |
| Synonyms | Tumor necrosis factor receptor superfamily member 12A; Fibroblast growth factor-inducible immediate-early response protein 14 (FGF-inducible 14); Tweak-receptor (TweakR); FN14; TNFRSF12A |
| Location | Membrane |
| Accession | Q9NP84 |
| Clone Number | S-3902 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | HeLa |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD266, also known as the TWEAK receptor (TWEAK-R) or fibroblast growth factor–inducible 14 (Fn14), is a ~14 kDa type I transmembrane glycoprotein of the TNF receptor superfamily (TNFRSF12A) that contains no death domain but is expressed at low levels in many normal tissues (heart, placenta, lung, muscle, pancreas) and at high levels on activated endothelial cells and multiple tumor lines, where it binds the cytokine TWEAK (CD255) and recruits cytoplasmic TRAF1, TRAF2 and TRAF3 to activate canonical and non-canonical NF-κB pathways, thereby regulating context-dependent processes including inflammation, endothelial proliferation and angiogenesis, hepatocyte growth during liver regeneration, and either survival or apoptosis of epithelial and malignant cells.
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