WB result of alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) Recombinant Rabbit mAb
Primary antibody: alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 40 kDa
alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) Recombiant Rabbit mAb (S-3355)
alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) Recombiant Rabbit mAb (S-3355)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) |
| Synonyms | Actin, aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein; ACTSA; ACTVS; ACTA2; Actin, gamma-enteric smooth muscle; Alpha-actin-3; Gamma-2-actin; Smooth muscle gamma-actin; ACTG2; ACTA3; ACTL3; ACTSG |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P62736、 P63267 |
| Clone Number | S-3355 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A431, HeLa, MCF7, NIH/3T3, C6, PC-12 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:4000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms, Rt |
Background
Alpha smooth muscle actin (alpha-SMA, acetyl E3) and ACTG2 (actin gamma 2, acetyl E3) are two important actin isoforms primarily involved in smooth muscle cell contraction and cytoskeletal maintenance. Alpha-SMA is a key component of the contractile apparatus in muscle tissues, particularly abundant in vascular, gastrointestinal, and urinary tract smooth muscles. Its acetylation modification (acetyl E3) may influence its function or stability. ACTG2 (γ2-actin) is a smooth muscle-specific γ-actin predominantly expressed in visceral smooth muscles, such as those in the intestines, and its acetylation (acetyl E3) also plays a role in cell motility and structural support. Studies indicate that both proteins can be detected by specific antibodies (e.g., Abcam's [E184] antibody) in immunohistochemistry, with α-SMA showing potential in inhibiting scar formation in fibroblast contraction assays. Their genes and protein structures are highly conserved, and they functionally cooperate in regulating mechanical contraction and cellular morphology in smooth muscle.
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Western Blot
WB result of alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) Recombinant Rabbit mAb
Primary antibody: alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 40 kDa
WB result of alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) Recombinant Rabbit mAb
Primary antibody: alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 40 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human smooth muscle. Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse smooth muscle. Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat smooth muscle. Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in C6 cells. Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
